Our main research interest is the regulation of gene expression at the level of transcription and chromatin / epigenetic. We are using a combination of in vitro (proteomics, genomics) and in vivo (leukemia mice models, xenotransplantation of human leukemic cells in immunodeficient mice) including single cell approaches (CyTOF and scRNAseq) to understand how hematopoietic stem cells differentiate into blood cells and how deregulation of this process causes leukemia.

Marjorie Brand Lab Website

M.A. Gillespie*, C.G. Palii*, D. Sanchez-Taltavull*, P. Shannon, W.J.R. Longabaugh, D.J. Downes, K. Sivaraman, H.M. Espinoza, J.R. Hughes, N.D. Price,T.J. Perkins**, J.A. Ranish** & M. Brand**. Absolute Quantification of Transcription Factors Reveal Principles  of Gene  Regulation in Erythropoiesis. Molecular Cell (2020), https://doi.org/10.1016/j.molcel.2020.03.03.031

C.G. Palii*, Q. Cheng*, M.A. Gillespie, P. Shannon, M. Mazurczyk, G. Napolitani, N.D. Price, J.A. Ranish, E. Morrissey**, D.R. Higgs** & M. Brand**. Single cell proteomics reveal that quantitative changes in co-expressed lineage-specific transcription factors determine cell fate. Cell Stem Cell (2019), 24:1-9 https://doi.org/10.1016/j.stem.2019.02.006

S. Fraineau, C.G. Palii, B. McNeill, M. Ritso, W.C Shelley, N. Prasain, A. Chu, E. Vion, K. Rieck, S. Nilufar, T.J. Perkins, M.A. Rudnicki, D.S. Allan, M.C. Yoder, E.J. Suuronen & M. Brand. Epigenetic activation of pro-angiogenic signaling pathways in human endothelial progenitors increases vasculogenesis. Stem Cell Reports, 9(5): 1573-1587, 2017

A. Benyoucef, C.G. Palii, C. Wang, C.J. Porter, A. Chu, F. Dai, V. Tremblay, P. Rakopoulos, K. Singh, S. Huang, F. Pflumio, J. Hebert, J-F. Couture, T.J. Perkins, K. Ge, F.J. Dilworth & M. Brand. UTX inhibition as a selective epigenetic therapy against TAL1-driven T cell acute lymphoblastic leukemia. Genes & Development, 30(5): 508-521, 2016

C.G. Palii, B. Vulesevic, S. Fraineau, E. Pranckeviciene, A.J. Griffith, A. Chu, H. Faralli, Y. Li, B. McNeill, J. Sun, T.J. Perkins, F.J. Dilworth, C. Perez-Iratxeta, E.J. Suuronen, D.S. Allan & M. Brand. Trichostatin A enhances vascular repair by injected human endothelial progenitors through increasing the expression of TAL1-dependent genes. Cell Stem Cell, 14(5): 644-657, 2014

C-P. Chaturvedi, B. Somasundaram, K. Singh, R.L. Carpenedo, W.L. Stanford, F.J. Dilworth & M. Brand. Maintenance of gene silencing by the coordinate action of the H3K9 methyltransferase G9a/KMT1C and the H3K4 demethylase Jarid1a/KDM5A. Proceedings of the National Academy of Sciences USA, 109 (46): 18845-50, 2012

C.G. Palii*, C. Perez-Iratxeta*, Z. Yao, Y. Cao, F. Dai, J. Davison, H. Atkins, D. Allan, F.J. Dilworth, R. Gentleman, S. Tapscott & M. Brand. Differential genomic targeting of the transcription factor TAL1 in alternate hematopoietic lineages. The EMBO Journal, 30 (3): 494-509, 2011  * contributed equally

C-P. Chaturvedi*, A.M. Hosey*, C. Palii, C. Perez-Iratxeta, Y. Nakatani, J.A. Ranish, F.J. Dilworth & M. Brand. Dual role for the methyltransferase G9a in the maintenance of ß-globin gene transcription in adult erythroid cells. Proceedings of the National Academy of Sciences, 106 (43): 18303-18308, 2009 * contributed equally

C. Demers, C-P. Chaturvedi, J. A. Ranish, G. Juban, P. Lai, F. Morle, R. Aebersold, F. Dilworth, M. Groudine and M. Brand. Activator-mediated Recruitment of the MLL2 Methyltransferase Complex to the ß-globin Locus.  Molecular Cell, 27: 573-584, 2007