The Ottawa Hospital Research Institute is the research arm of The Ottawa Hospital – one of Canada’s largest learning and research hospitals. We are proudly affiliated with the University of Ottawa and supported by The Ottawa Hospital Foundation. With more than 2200 scientists, clinician investigators, trainees and staff, and total revenues of more than $120 million, we are one of the foremost Canadian hospital-based research institutes. Our research spans more than a hundred different diseases, conditions, and specialties with an overall focus on translating discoveries and knowledge into better health.
Job Description
We are looking for a knowledgeable and enthusiastic Masters or PhD student in the Department of Cellular & Molecular Medicine to join our research team at the OHRI. Our research focuses on understanding what makes endothelial cells functionally specialized for each organ in the body. We specifically focus on the liver and its liver sinusoidal endothelium as this knowledge will help build new cell-based therapies and therapeutics for liver disease, liver cancer, and hemophilia A. The Gage lab’s research extensively uses mammalian cell culture of human pluripotent stem cells to model early human development and create endothelial progenitors that we transplant in animal models where they functionally mature. Using CRISPR-Cas9, lentiviral gene vectors and functional genetics, we can directly link genes to disease related functions. These links are validated with many molecular and computational techniques including (single-cell RNAseq and ATAC-seq, RT-qPCR, immunofluorescence, electron microscopy, ELISA, ligand binding assays, flow cytometry, magnetic and fluorescence-based cell sorting, etc.)
Basic Requirements (Education/Experience):
• Experience in mammalian cell culture (cell lines or preferably human pluripotent stem cells)
• Experience in Molecular Biology (RT-qPCR, immunohistochemistry, immunocytochemistry)
• Experience in quantitative Cell Biology (Flow cytometry, FACS, microscopy)
• Experience with animal models (mice).
• Excellence at the academic level and in research
• Excellent written and oral communication skills
Preferred Qualifications:
• Experience with bioinformatics analysis (programing in R: analysis of bulk RNAseq or single-cell RNAseq)
• Experience working with lentivirus
• Eligibility for CIHR/NSERC graduate student scholarship applications (Canadian citizens or Permeant residents)
Contract Details:
This is a PhD 5-year or MSc 2-3 years full-time contract with the possibility of renewal
Stipend: Minimum Biweekly Stipend for PhD $1,030.00 and for MSc $911.153
Comments to Applicant:
Individuals who are interested in the position should submit curriculum vitae, a copy of official transcripts, statement of research interest and names of three references to:
Contact Info:
Name: Dr. Blair Gage
Title: Scientist
Program: Regenerative Medicine Program
Ottawa Hospital Research Institute
Address: 501 Smyth Road, Ottawa, ON, K1H 8L6
Email: bgage@ohri.ca
Website: https://www.ohri.ca/profile/bgage/gage-lab
The Ottawa Hospital Research Institute is committed to building and sustaining a welcoming and supportive culture that promotes equity, diversity and inclusion for trainees, researchers, and staff. We are devoted to identifying and removing barriers that limit equitable access to opportunities within our organization. The Ottawa Hospital Research Institute strongly supports initiatives that encourage the participation of all groups, in particular the four groups defined in the Employment Equity Act (women, Indigenous peoples, persons with disabilities and members of visible minorities).
We thank all applicants for their interest. However, only those invited for an interview will be contacted. The Ottawa Hospital Research Institute is an equal-opportunity employer. Upon request, accommodations due to a disability are available throughout the selection process.
