Technologies

Experimental Approaches:


1) Genetic modification of neural progenitors.

The lab has expertise in a number of techniques to manipulate retinal progenitors both in vivo and in culture, including mouse genetics, retroviral transduction, and electroporation. Developing retinas can be explanted and grown in tissue culture, which facilitates a variety of experimental approaches.

Cone derived from progenitors transfected with green fluorescent protein at embryonic day 14.5 via in utero retinal electroporation, and allowed to develop until postnatal day 21. Cone opsin (cyan) and peanut agglutinin (magenta) mark the cone photoreceptors.




Clonal analysis allows the complete lineage generated by individual progenitor cells to be analyzed. Here, retroviral clones (green) generated from dividing progenitors transduced at postnatal day 0, and harvested 2 weeks later. Vsx2 protein staining marks bipolar cells (red), while Hoechst stains the DNA (blue), allowing the tissue to be visualized in full. The clone on the left contains 3 rods, while the clone on the right contains a rod and a bipolar cell.





2. Proteomics

We perform Bio-ID on primary retinal cultures, as well as co-IP/MS on tissue. 





3.  Genomics and transcriptomics

We use cut&run-seq and ATAC-seq to examine how nucleosome remodelling complexes interact with the genome.

We use multi-seq Multi-seq to perform multiplexed single cell RNA-seq. See Clemot-Dupont et al.