Flow Cytometry and Cell Sorting

Instrumentation: Cytek Aurora

Cytek Aurora
Location: The Ottawa Hospital Cancer Centre, 3rd floor, South Wing, room 3325E, 501 Smyth Road

A prodigy incorporating a unique combination of innovative technologies that takes flow cytometry to the next level of performance and flexibility.

With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora system suits every laboratory’s needs, from simple to high-complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution.

Optics specifications:

The Cytek Aurora is equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, 640nm), allowing the researchers to detect up to 40 different demonstrated fluorophores. Due to its large detection capability, there is no need to change the configuration of the optical filters.

Deep immunoprofiling:

The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 colors all the way to 40 colors.

DOI: 10.1002/cyto.a.24213. OMIP-069: 40 Color Deep Immune Profiling

Automated Sample Loader

The ASL offers more versatility when running your samples at high-throughput. In addition to acquisition from 96-well plates, the ASL is compatible with 96-deep well plates and 40-tube racks. For each carrier type, Cytek has provided preset mixing speeds and frequencies, which are also fully customizable to meet your individual experimental requirements. The ASL is designed to streamline experimental workflows and integrates seamlessly into the Aurora system

Autofluorescence extraction

The Aurora system’s implementation of full spectrum flow cytometry enables the use of autofluorescence extraction to further improve data clarity. Certain sample types, such as yeast and tumor samples, present the challenge of high autofluorescence. For these challenging applications involving highly autofluorescent particles, let the software's autofluorescence extraction tool bring new levels of resolution.