Flow Cytometry and Cell Sorting
Laboratory services
Flow cytometry
- Immunophenotyping/immunofluorescence by monoclonal antibodies.
- Total/Absolute population counts.
- Analysis of surface and intracellular fluorescent labelling, simultaneously.
- Monitoring gene transfer and expression using fluorescent proteins.
- Simultaneous detection and sorting of GFP and YFP.
- DNA content and cell cycle analysis using PI, Hoechst, BrDU, etc.
- Hoechst 33342 side population.
- Cell viability with exposure to drugs, toxins, etc.
- Apoptosis.
- Caspase activity.
- Telomere length measurement.
- Neutrophil functions (phagocytosis and oxidative burst).
- NK cytolytic activity.
- MLR / CTL cytotoxicity.
- Caspase activity.
- Mitochondrial function (DiO, JC-1)
- Intracellular Calcium Flux.
- Q-Prep whole blood lysis.
Cell Sorting
- High-speed cell sorting.
- Cell sorting of one to four subpopulations.
- Large particle sorting
- Single cell deposition in multi-well plates.
Antibody Testing and Compensation matrix
- Additional service is offered for antibody testing of mouse, rat and hamster origin using antibody capture beads. This beads are a useful tool to detect the intensity of your antibodies and to calculate the compensation matrix in experiments with more than 6 parameters.
Training
- Flow cytometry basic training is intended for students, technicians and investigators wanting to strengthen their background and understanding of flow cytometry. The 2-hour session will provide the participants with the required tools for setting up successful flow cytometry experiments.
- Specific Flow Cytometry training is offered on an as-needed basis; please contact the facility coordinator for further details.
