Mass Spectrometry Service
Studying protein-protein interactions and their aberrant expression is essential for unravelling the complexities of cellular processes, disease mechanisms, and drug development; making proteomics indispensable in advancing biomedical science. The LC-MS Proteomics Core Facility leverages the high-resolution and sensitive capabilities of the Orbitrap Fusion Lumos mass spectrometer. The facility specializes in maximizing the identification of proteins in your sample, determining their relative quantities, and the characterization of post-translational modifications.
The Proteomics Core can accept samples in a variety of formats
- digested peptides in solution and ready for LC-MS analysis
- proteins in solution (such as from cell lysates)
- purified proteins in SDS-PAGE gel bands
And Core can analyze them in a variety of ways:
- general detection of the proteins present in your sample
- label-free quantitation of the detected proteins (label-based quantitation strategies are also welcomed)
- discovery of post-translational modifications (PTMs) and other proteoforms
- targeted detection of a few proteins of interest in an antibody-free manner
- customization of sample preparation and analysis strategies
Please reach out to Andrew Macklin at proteomics@ohri.ca for a consultation on your unique project’s needs. If submitting gel bands, please review the submission guidelines and gel band excision protocol. After consultation, you can complete the Service Request form and submit it along with your samples to:
Proteomics Core Facility
Room W5256 (Critical Care Wing),
The Ottawa Hospital, 501 Smyth Road,
Ottawa, Ontario, K1H 8L6, Canada.
Your samples will be analyzed by LC-MS and you will be notified by email when your results are ready.