High Content Imaging Core
Operetta CLS live
The Operetta is a high throughput microplate imager for high-content analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images. The combination of high power LED excitation with fast, precise mechanics and one large format sCMOS camera enables fast imaging. Controlled by Harmony software, it offers a seamless workflow, for reliable discrimination of phenotypes even in complex cellular models.
Manufacturer Website
The High Content Imaging Core possesses an Operetta CLS Live system, equipped with a live cell chamber, a confocal unit and fully automated water immersion objectives. With a 8-LED kit and the Harmony software, the Operetta CLS Live allows the creation of fully customized analyses suited for any experimental design.
Operetta CLS Live specs
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Illumination
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8 LED kit (365nm, 440nm, 475nm, 510nm, 550nm, 580nm, 630nm, 660nm)
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Camera
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4.7 Mpx sCMOS (2160x2160 pixels at 6.5 µm/pixel)
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Imaging
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Brightfield, DPC, Widefield and Confocal
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Objectives
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Objective turret (regular range: 5-20x, water immersion range: 20-40x)
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Focus
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Fast laser-based autofocus
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Live cell
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Live-cell imaging chamber (temperature and CO2 control)
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Machine learning
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PhenoLOGIC algorithm
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Acquisition
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PreciScan intelligent acquisition plug-in
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Software
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HCS Studio software for integrated data collection and analysis
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Operetta applications
The Harmony software allows for virtually any application, designed by the user. Our core facility offers full training, 24/7 access, experimental support as well as technical support.
If you have any questions, please Contact Us.
