Human Pluripotent Stem Cell Facility
Equipment
Core equipment
Core users are charged an hourly fee ($15 per hour) for access to all consumables and instruments required to culture and maintain healthy hPSCs. The fee includes the use of the following equipment:
- Biological safety cabinets (4)
- Tri-gas incubators set to Hypoxic (3), normoxic (2), and sensitive cell incubators (1). These incubators monitor and maintain O2, CO2, humidity, and temperature that allows for consistent, reliable culturing conditions that most closely resemble in-vivo microenvironments.
- Dissecting microscopes under Laminar flow hoods for convenient cell cleaning and manipulation. Users can manually select pluripotent colonies while maintaining sterility.
- Controlled rate freezer to maximizes cell recovery, saving time and money down stream.
- Automated cell counter (Countess 3) with reusable slides, significantly reducing waste.
- 24/7 access and personal storage space at room temperature, 4⁰C and -20⁰C.
Additional equipment
Lonza Nucleofector 4D Core & X-unit
The NucleofectorTM Technology uses a specific combination of optimized electrical parameters and cell type-specific solutions which enables transfer of a molecule directly into the cells’ nucleus. Due to this independency from cell proliferation, it allows for efficient transfection of even non-dividing primary cells (like resting T cells or neurons) and speeds up expression. For specifications, documentation, eduction and other information, see the Lonza Bioscience website.
Usage fees
| Internal academic | External academic | Industry and government |
| $40 per session | $60 per session | $200 per session |
Covaris
Versatile shearing device for DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. For specifications, features, protocols and other information, see the Covaris website.
Usage fees
| Internal academic | External academic | Industry and government | 150 µL tubes |
| $25 per hour | $40 per hour | $100 per hour | $15 |
Neon Transfection System
The Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. For specifications, user guides and other information, see the Thermo Fisher Scientific website.
Usage fees
| Internal academic | External academic | Industry and government |
| $40 per 2-hour session | $60 per 2-hour session | $100 per 2-hour session |
| Neon tips | Neon cuvette | Technician time |
| $15 | $56.01 | $50 per hour |
NanoPRO 1000
A simple Western system for charge-based assays to characterize proteins. See the Bio-Techne website for more information. Contact us for pricing.
ThermoFisher Arrayscan VTI (Cellomics)
The Thermo Scientific™ ArrayScan™ XTI Live High Content Platform is a complete research solution for automated cellular imaging analysis. See the Thermo Fisher Scientific website for more information. Contact us for pricing.
Roche Light Cycler 480
The LightCycler 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. See the Roche website for more information. Contact us for pricing.