Research Services
- Training on human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs) culture methods (harvesting, passaging, thawing and freezing)
- Training for reprogramming (differentiation, characterization)
- Perform as a service (cell culture, reprogramming, differentiation, characterization, CRISPR genome editing)
- Primary cell isolation extraction and expansion
- Teratoma injection and extraction
- Protocol development
Laboratory Services Pricing (effective as of January, 2021)
|
Service
|
Fee if facility does work for uOttawa / affiliate lab
|
Fee if user from uOttawa / affiliate lab does work
|
Fee if facility does work for external client (other academic institutions, government or private)
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| Training (in person or virtual)
|
$50/hr
|
$50/hr
|
$65/hr
|
| Technician time
|
$40/hr
|
None applicable
|
$75/hr
|
| Reprogramming
|
$750/sample
|
None applicable
|
$1200/sample
|
| Harvesting/freezing
|
$20/sample
|
None applicable
|
$40/sample
|
| Differentiation
|
Value is variable due to length of experiment
|
None applicable
|
Value is variable due to length of experiment
|
| Characterization and analysis (Cellomics)
|
$150/12 well plate
|
None applicable
|
$300/12 well plate
|
| Teratoma assay (animal housing, cell injection and teratoma harvesting)
|
$650 per injection
|
$650 per injection
|
$1000 per injection
|
| Consumables
|
$400/month
|
$400/month
|
$600/month
|
If you would like to request a service that is not outlined in this page, please email hpscf@ohri.ca, and we would be happy to provide a customized quote for you. For any questions or feedback, please contact us at hpscf@ohri.ca.
Differentiation
We have successfully differentiated the following cell types (see below) from iPSCs and/or ESCs. Training on how to differentiate iPSCs and ESCs is available at the facility. Prices vary depending on the protocol used for differentiation.
- Smooth Muscle Cells
- Vascular Smooth Muscle Cells
- Chondroprogenitors Cells
- Cardiomyocytes
- Neuron Progenitor Cells (NPCs)
- Neurons
- Neural Crest Cells
CRISPR/Cas9 genome editing
We have developed the protocols necessary to perform edits in pluripotent stem cells using a number of different strategies. For instance, in addition to relying on random insertions/deletions through non-homologous end joining (NHEJ) to knockout a gene of interest, we can pair a Cas9/sgRNA vector with a single stranded DNA template for precision knockout. We have also successfully inserted large transgenes containing selection markers to knockout your gene of interest. We also have all of the tools and vectors needed for gene knock-in at the AAVS1 safe harbor locus for sustained expression. Please contact for more information.
