Proteomics Core Facility
Equipment
Orbitrap Fusion Lumos (ThermoFisher Scientific)
High-performance system that leverages both the high-resolution Orbitrap detector and the fast, sensitive linear ion trap detection.
Versatile with HCD, CID and ETD peptide fragmentation techniques to maximize sequence coverage.
Nanospray ionization maximizes sensitive detection of peptides from low input samples.
UltiMate 3000 RSLC nano HPLC (Dionex)
High-performance liquid chromatography system.
Nanolitre per minute flow rates.
Multidimensional chromatography (SCX + RP) is an option.
Data Analysis
Protein identification and quantitation is typically performed by matching observed spectra to protein sequences using Fragpipe software.
Maxquant and Mascot (in-house server) are also available upon request.
Further data analysis and visualization is performed with Fragpipe-Analyst, PDV Viewer, We also have a Scaffold license so that clients can navigate their results with the free viewer.