Proteomics Core Facility

Proteomics Core Facility

Equipment

Orbitrap Fusion Lumos (ThermoFisher Scientific)

  • High-performance system that leverages both the high-resolution Orbitrap detector and the fast, sensitive linear ion trap detection.

  • Versatile with HCD, CID and ETD peptide fragmentation techniques to maximize sequence coverage.

  • Nanospray ionization maximizes sensitive detection of peptides from low input samples.
     

Orbitrap Fusion Lumos MS

UltiMate 3000 RSLC nano HPLC (Dionex)

  • High-performance liquid chromatography system.

  • Nanolitre per minute flow rates.

  • Multidimensional chromatography (SCX + RP) is an option.

Ultimate 3000 HPLC

Data Analysis

Protein identification and quantitation is typically performed by matching observed spectra to protein sequences using Fragpipe software. 

Maxquant and Mascot (in-house server) are also available upon request.

Further data analysis and visualization is performed with Fragpipe-Analyst, PDV Viewer,  We also have a Scaffold license so that clients can navigate their results with the free viewer.

Fragpipe logo