StemCore Laboratories
Bulk Molecular Analysis
Overview
You might be wondering why we don't just call this "Bulk Sequencing" - it's because this service encompasses not just sequencing (RNA, DNA, epigenetics) but also proteomics via our Olink platform. Bulk molecular analysis captures the full scope.
Bulk molecular analysis examines the aggregate signal from entire cell populations within your samples, providing average measurements across all cells rather than single-cell resolution. Whether you're studying gene expression patterns across conditions, investigating chromatin architecture, or quantifying protein levels in samples, these high-throughput methods reveal molecular differences, regulatory mechanisms, and biological pathways.
On this page:
Applications We Support
RNA Sequencing
- mRNA-seq (poly-A selection)
- Total RNA-seq (ribosomal depletion)
- Small RNA and miRNA profiling
- Long non-coding RNA (lncRNA) analysis
Proteomics
- Olink Reveal for high plex (~1000 targets) protein quantification from small quantities of diverse sample matrices (plasma, urine, BAL etc.)
Chromatin & Epigenomics
- ChIP-seq (chromatin immunoprecipitation sequencing)
- CUT&RUN (cleavage under targets and release using nuclease)
- ATAC-seq (assay for transposase-accessible chromatin)
- Hi-C (chromosome conformation capture)
- Methylation sequencing
Specialized Applications
- TSO 500
- Ampli-Seq and other targetted panels
WORKFLOW
- Contact Caroline to discuss your research question and sample types
- Receive protocol recommendation and sample requirements
- Submit request via Idea Elan Infinity (https://secure17.ideaelan.com/uOttawa/Public/applogin.aspx)
- Submit samples for library preparation
- Libraries sequenced on NovaSeq X (see our NGS page for sequencing details)
- Data delivered via BaseSpace or electronic transfer
OUR APPROACH
Library preparation protocols vary significantly by application. We work with you to select the appropriate method based on your biological question, sample type, and input material.
Caroline will recommend the best protocol and provide specific sample requirements for your project.
PRICING
| Application | Price per Sample (Internal) |
| mRNA-seq (poly-A selection) | Enquire |
| Total RNA-seq (ribosomal depletion) | Enquire |
| Small RNA/miRNA profiling | Enquire |
| Complete RNA (mRNA and miRNA) | Enquire |
| Olink Reveal proteomics | Enquire |
| ChIP-seq | Enquire |
| CUT&RUN | Enquire |
| ATAC-seq | Enquire |
| Hi-C | Enquire |
| TSO500 | Enquire |
| AmpliSeq | Enquire |
Library preparation pricing varies by protocol complexity. Contact Caroline for detailed quotes.
Pricing shown is for internal users (OHRI/UofO). Contact for external pricing.
Sequencing costs are separate - see our NGS page for flow cell pricing.
GETTING STARTED
Contact:
Caroline Vergette, NGS Specialist
Email: cvergette@ohri.ca
Phone: 613-737-8899 ext. 73251