StemCore Laboratories
Next Generation Sequencing
On this page:
Overview
Next Generation Sequencing (NGS) uses massively parallel sequencing to analyze millions of DNA or RNA fragments simultaneously, enabling comprehensive genomic and transcriptomic studies at unprecedented scale and speed. From sequencing entire genomes to quantifying gene expression across thousands of genes, NGS has transformed biological research by making large-scale sequencing accessible and cost-effective.
Our Illumina NovaSeq X, installed in 2025, delivers high-throughput sequencing for projects ranging from whole genome analysis to targeted applications. We offer flexibility to purchase full flow cells for fastest turnaround or submit individual samples for shared runs.
Note: NGS sequencing is included in our other service workflows (Single Cell Analysis, Population-Omics, Spatial Biology). If you need library preparation or specific assay services, please see those pages first.
Finding the Right Service for You
Looking for bulk RNA-seq, ChIP-seq, or epigenomics applications?
→ See our Population-Omics page for library preparation and application-specific workflowsNeed single-cell analysis?
→ See our Single Cell Analysis page for scRNA-seq, scATAC-seq, and multiomeHave pre-made libraries ready to sequence?
You're in the right place.Need whole genome or exome sequencing?
We provide complete WGS and WES services - you're in the right place.
Equipment
Illumina NovaSeq X
- XLEAP-SBS chemistry: Illumina's latest sequencing technology with improved surface chemistry delivering higher cluster density, better signal-to-noise ratio, and superior data quality
- Flexible throughput: Three flow cell options (1.5B, 10B, or 25B reads) to match your project scale
- Maximum output: Up to 8Tb per run on the 25B flow cell
- Read configuration: 2x150bp paired-end reads
- Fastest turnaround: Reduced run times compared to previous generation platforms
- Scalability: From pilot projects to population-scale studies on a single platform
What We Offer
Pre-Made Library Sequencing
Submit your prepared libraries with barcode information - we perform QC, quantification, and sequencing
Whole Genome Sequencing (WGS)
Complete service from DNA to data - we handle library preparation and sequencing
Whole Exome Sequencing (WES)
Complete service from DNA to data - we handle capture, library preparation, and sequencing
Other Applications
For RNA-seq, ChIP-seq, ATAC-seq, and other library prep workflows, see our Population-Omics page
Pricing
| Flow Cell Type | Lanes | Full Flow Cell | Per Lane |
| 1.5 Billion Reads | 2 | from $3655 | from $1827 |
| 10 Billion Reads | 8 | from $12197 | from $1525 |
| 25Billion Reads | 8 | from $27360 | from $3454 |
| Service | Cost per sample | Reads | Coverage |
| Whole Exome Sequencing | from $204 | 20M | 100x |
| Whole Genome Sequencing | from $494 | 300M | 30x |
Pricing shown is for internal users (OHRI/UofO). Contact for external pricing.
Master Service Agreement required for external clients.
Getting Started
Contact:
Caroline Vergette, NGS Specialist
Email: cvergette@ohri.ca
Phone: 613-737-8899 ext. 73251