StemCore Laboratories
Single Cell Analysis
On this page:
Overview
Single-cell analysis resolves cellular heterogeneity by measuring gene expression, chromatin accessibility, or protein abundance in individual cells rather than averaging across bulk populations. This approach reveals rare cell types, identifies cellular states, maps developmental trajectories, and uncovers cell-to-cell variability that drives biological processes.
Equipment
10x Genomics Chromium X
The Chromium X offers unparalleled flexibility in single-cell analysis, supporting everything from small-scale targeted experiments to high-throughput comprehensive profiling. On-chip multiplexing (OCM) enables significant cost savings by allowing multiple samples to run together on a single chip, splitting standard 20k cell samples into 4x 5k cell samples per channel - ideal when researchers don't need full 20k cell depth.
We offer the complete 10x Genomics workflow portfolio.
Applications We Support
10x Genomics Workflows:
- scRNA-seq 3' (standard gene expression profiling)
- scRNA-seq 5' (full-length transcript analysis, V(D)J profiling compatible)
- Flex (targeted gene panel approach)
- scATAC-seq (chromatin accessibility)
- Multiome (simultaneous gene expression and chromatin accessibility)
- Feature barcoding (protein quantification alongside RNA)
- On-chip multiplexing (OCM) - run multiple samples together for cost savings
Other Single-Cell Platforms:
- We are trained and equipped to support additional single-cell technologies:
- Illumina single-cell protocols
- Parse Biosciences workflows
- Singleron protocols
Contact Kathy to discuss which platform best suits your research needs.
Workflow
- Contact Katayoun to discuss your application and sample types
- Receive sample requirements and protocol recommendations
- Submit request via Idea Elan Infinity (https://secure17.ideaelan.com/uOttawa/Public/applogin.aspx)
- Submit samples
- Library preparation and sequencing (sequencing included in pricing)
- Data delivered via BaseSpace or electronic transfer
Pricing
All prices include library preparation and sequencing to 10x specifications.
| Application | Price From (Internal) |
| scRNA-seq 3' | Contact for quote |
| scRNA-seq 5' | Contact for quote |
| Flex | Contact for quote |
| scATAC-seq | Contact for quote |
| Multiome | Contact for quote |
| Feature Barcoding | Contact for quote |
| On-chip multiplexing (OCM) | Contact for quote |
| Other platforms (Parse, Singleron, Illumina) | Contact for quote |
Pricing shown is for internal users (OHRI/UofO). Contact for external pricing.
Getting Started
Contact:
Katayoun Sheikheleslamy, Single Cell & Spatial Biology Specialist
Email: ksheikheleslamy@ohri.ca